Following glutaraldehyde fixation, cultures were washed thrice in 0.1 M cacodylate buffer, fixed for 1 h with 1% osmium tetroxide, washed in buffer, dehydrated with ethanol and embedded in Epon 812 resin (TAAB, Berks, UK).
After fixation, cultures were permeabilized with 0,1% Triton X-100 and immunofluorescence was performed in phosphate buffered saline (PBS), 1% normal goat serum (NGS), 0,1% Triton X-100 and 0,2 mg/ml sodium azide.
After fixation, cultures were washed three times with PBS-w and permeabilized with 0.1% Triton-X-100 for 5 min; then washed and blocked with 1% (w/v) BSA in PBS-w for 30 min.
This organism uses a Mo-based nitrate reductase during nitrate utilization and a Mo-based dinitrogenase during N2 fixation under culture conditions here.
Cell cultures were fixed with a 4% paraformaldehyde in phosphate buffer containing 4% sucrose for 20 min. After fixation, the cultures were permeabilized and blocked with 5% fetal bovine serum in PBS containing 0.05% TritonX-100.
After fixation co-cultures were permeabilsed using ice-cold methanol, blocked with 10% rabbit serum followed by 0.3% hydrogen peroxide.
In this study, we examined the fractionations associated with Mo assimilation during nitrate reduction and N2 fixation in cultures of the freshwater cyanobacterium Anabaena variabilis ATCC 29413.
Fashion came next, which, like his photography today, exhibited an intense fixation on rock culture.
I also believed that we'd be over our nostalgic fixation on analog culture and its totems very quickly.
The modern fixation with speakeasy culture can be traced to Milk & Honey, a clandestine bar that Sasha Petraske opened in 2000, behind an unmarked door with an unlisted phone number on the Lower East Side.
