Immunocytochemistry, however, on epoxy-embedded sections is difficult due in part to the plastic itself and to the fixation conditions.
Furthermore, it has been revealed that subtle adjustment in fixation conditions with, e.g., PFA condition, can have dramatic effects on the immobilization of molecules within cellular membranes [12].
Such findings are in excellent agreement with previous observations that molecules in cellular membranes can remain mobile under certain, relatively mild PFA fixation conditions [12].
The loading, bone quality, and acetabular cup fixation conditions were found to have only minor effects on the predicted contact pressure distribution along the bearing surfaces.
Two fixation conditions were used for the whole-mount immunohistochemistry: Bouin's [86] and methanol/acetone.
It should be noted that we did not analyze a large set of fixation conditions in testing these monoclonals.
There were also significant differences between the eyes-open with and without fixation conditions (i.e., EO-F vs. EO).
Overall, it was impossible to interfere with- and to obtain detailed information on fixation conditions for the majority of the tissues examined.
First, any perceptual biases (for example, horizontal vs. vertical illusion effects) not related to saccadic movement are controlled by our fixation conditions.
Prior studies have shown no clear patterns of expression of Lrp5 and -6, depending upon the antibody reagents used, the assay, and the fixation conditions.
Under the best fixation conditions, the membrane features of fixed cells were kept closest but not identical to their natural state of live cells.
