Sentence examples for fixation and processing of from inspiring English sources
The detection of NANOG expression in hESCs has depended on methods which require fixation and processing of cells, which is labor-intensive and prevents the detection of NANOG expression in real-time in viable cultures.
The current formalin-based methods of fixation and processing of tissue for histopathological evaluation hinder the reliable analysis of macromolecules.
Pre-analytic variables in the fixation and processing of breast specimens are well described to undermine HER-2 results.
Microwave fixation and processing of cell monolayers for transmission electron microscopy was described in detail elsewhere [43].
Next we investigated immunohistochemical staining of phospho-CRMP2 and phospho-tau after the primary neurons were embedded in paraffin to accurately mimic the fixation and processing of human clinical tissues used in diagnostic histopathology (Fig. 3b).
In addition, the preservation of the antigenic site(s) during tissue (cell) fixation and processing of the tissue is an important factor which may have an influence on the immunopositive/immunonegative results.
Only few studies have adequately addressed analytical aspects of fixation and processing on array CGH.
(A) ACHN cells in 384-well black wall, clear bottom plates were treated with rocaglamide for 4 h (circles) or 24 h (squares) followed by labeling with puromycin (10 µg/mL final concentration for 30 min), fixation, and processing for ICW analysis of puromycylated protein (see Materials and Methods).
For labeling of surface nucleolin, cells were incubated with mAb D3 for 45 min at room temperature before PFA fixation and processing for confocal immunofluorescence microscopy.
Also, incorporation of DG into glycogen, glycoprotein and glycolipid would result in labelled metabolites that are resistant to washout during fixation and processing to identify cell types.
As shrinkage due to tissue fixation and processing is typical of histological processing [ 27, 28], with different tissues affected to different degrees [ 29], the histological image is used here to provide an understanding of the biological features, rather than to validate the geometrical distance and orientation of the features found in the OCT images, which were acquired in fresh tissue.
