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The five-minute gun.
It has a 20-minute wash for 1.5kg.
After a five minute wash in 1 mM Ca, application of Calindol (10 µM) resulted in a F/F0 rise of 0.269±0.017 in the same 12 cells (Figure 5A).
Two final washes (ten minutes, then five minutes) in 1× TBS-T and one five minute wash in 1× TBS were performed.
The coverslips were then washed two times five minutes in PBS followed by a five minute wash in PBS containing Hoechst (5µg/µl, Sigma-Aldrich) before being mounted using Fluorescence Mounting Medium (Dako) and stored at 4°C.
After ten minutes, wash off.
After her 6-3, 6-3 loss to 16th-seeded Nathalie Tauziat, the first Frenchwoman to reach a Wimbledon semifinal since Francoise Durr in 1970, Davenport allowed herself a private two-minute crying jag and then washed away her tears in the shower.
Forty-eight hours after siRNA transfection, cells were pulse-labeled with the thymidine analogue IdU for 30 minutes, washed and labeled with CldU for further 30 minutes15,16.
Table 4 shows a comparison of the 1st through 89th minute TAC GDC models with 5th through 89th minute TAC washout models.
Instead of a two-minute bin route, Mr Kitching would have been forced to push his bin 200 yards, dodging trees, fences and washing lines if plans to convert Old Blacksmith's Yard, next door to his house, had succeeded.
By the 50th minute they had equalised.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com