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PERRI CESARELLI gets up at 5 30 a.m., takes the dog out, eats breakfast, showers, dresses, then, at 7 05 walks the 12 minutes to the train station in Little Neck, N.Y., leaving herself a five-minute buffer before catching the 7 22 into Manhattan.
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The five-minute gun.
QCM experiments performed with NTA-NO2 functionalized crystals consist of the following steps: 1) Buffer A as baseline (50 mM Tris HCl (pH 7.4), 300 mM NaCl), 2) 10 minutes 10 μM His6-GFP in Buffer A, 3) 10 minutes Buffer A, 4) 20 minutes UV-light with Buffer A, 5) 10 minutes Buffer A after UV exposure.
You can take a trip downtown at lunchtime without a 20-minute buffer to find parking.
However, you can't help feeling that the 60-minute buffer was put there to lower expectations.
There is a 30 minute buffer for live radio.
When scheduling an appointment or meeting, allow yourself at least a 30-minute buffer.
St Kilda regained the lead in the 14th minute of the first term and never relinquished it, although their buffer was in single figures for most of the opening half.
David Nofoaluma put the Tigers ahead again in the 31st minute when he forced his way through three Cowboys defenders and another Moses penalty goal two minutes from the break gave the home side a six-point buffer at half-time.
By the 50th minute they had equalised.
He was beaten in the 61st minute.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com