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After the five week experiment, 60% of impaled items were still present on territories.
The initial density of grazers probably did not determine final grazer densities since the grazers reproduce at high rates and likely reached carrying capacity in the mesocosm tanks by the end of the five week experiment [54].
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At the beginning of the experiment, all individuals were < 75 μm, at the end of the eight week experiment the > 90 μm fraction was analysed.
These differences in δ13C and δ18O values are attributed to very strong Rayleigh fractionation and minimal evaporation occurring over the six week experiment in these 'closed' systems (test tubes).
So did we learn anything from the burden of this four week experiment?
Data were collected in minute bins throughout the one week experiment.
For the duration of the four week experiment, corals were supplied with 3.5 hours of shaded light (30 36 µmole photons m−2 s−1) followed by 5 hours of un-shaded light (87 107 µmole photons m−2 s−1), followed by another 3.5 hours of shaded light and 12 hours darkness each day to approximate their natural diurnal light cycle.
For chronic toxicity eight week experiment was designed.
For the four week experiment, no selection was employed; homozygous T4 seeds were germinated directly on peat moss.
The subsequent damping down of the transcriptional wave suggests that the acclimation process was concluded by the end of the two week experiment.
At the end of the eight week experiment, samples of fasting blood were collected by jugular venipuncture using heparin or EDTA.
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