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The pellet was resuspended in five volume of ZnSO4 solution, thoroughly mixed using vortex mixer and exhaustively examined under light microscope.
Then cells were harvested and lysed in five volume of lysis buffer (25 mmol/L Tris-HCl, 150 mmol/L NaCl, 3 mmol/L MgCl2, 0.5% NP-40, 1 mmol/L DTT, 5% Glycerol, 1% PI, pH 7.5) for rotating at 4°C for 2 h, followed by centrifugation at 21,000 ×g for 30 min.
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"Five volumes of the Encyclopedia Brit. on his sofa.
Sontag's reputation rests on the five volumes of nonfiction she published between 1966 and 1980.
Five volumes of the proposed 70 have been published so far.
Yes, Virginia Woolf's fiction is difficult, but her six volumes of letters, five volumes of diaries and countless volumes of literary criticism convey, from a very early age, dazzling humor, insight and humanity.
Five volumes of buffer and one volume of 111InCl3 (Covidien, Petten, The Netherlands) were added.
He wrote feverishly, finishing five volumes of poetry and autobiography.
Between 1909 and 1941 he wrote thirty-six novels, five volumes of short stories, two original plays and three volumes of memoirs.
The first five volumes of each fMRI run were discarded.
The beads were washed with five volumes of nucleus extraction buffer with 350 mM NaCl, followed by five volumes of low-salt extraction buffer (150 mmol/l NaCl).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com