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GFP expression was maintained after five passages in HeLa cells.
Five passages were chosen to permit assessment of literary reading materials (e.g., short stories, narrative extracts, and traditional tales).
The other five passages were designed to assess comprehension of informational text (e.g., expository passages, instructions, and manuals).
hMSCs were treated with Ca2+/hypoxia for five passages in the absence or presence of U0126 or BAPTA-AM.
No undesired genes were detected either before or after five passages of LV1 or LV2B in eggs.
We showed that cell deregulation/dedifferentiation was reversible by treating the cell monolayers, after five passages with streptozotocin (STZ), a well-known β-cell toxin.
Eighteen clones derived from single DFCs were harvested; however, only three clones were amplified successfully more than five passages and 90 95 days in culture.
Cells were labeled over the course of five passages in the labeling medium prior to analysis.
All MEFs used in this study were less than five passages.
Confluent monolayers were trypsinized twice a week and used for a maximum of five passages.
All experiments with normal bladder cells were performed within five passages.
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