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Five gram sample was examined by binocular magnifier (20×).
Five gram of each soil sample was inoculated in 100 ml conical flask containing sterile nutrient broth (NB) supplemented with 0.1% of tryptophan [18].
Five gram of homogenized samples were spiked with 5 μL of internal working standard solution (10 μg/mL) and vortexed for 1 min to mix thoroughly.
They consisted of five Gram-positive and five Gram- negative strains (Table 1).
Soil sample (five gram) was suspended in 50 mL of phosphate buffered saline (1x).
Five gram of 5-AZA-CdR powder (Sigma, USA) was dissolved in 2 mL FBS-free EGM-2 solution.
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Espresso pods are filled with around five grams.
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