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For instance, two plasmids carrying the same colE1 ori yet different antibiotic markers - kanamycin versus ampicillin - were found to be 100% compatible after five consecutive transfers under just kanamycin selection [ 34].
Even though it is missing several genes involved in biotin synthesis (e.g., bioA, bioF, and bioW), A. succinogenes grows repeatedly in the absence of biotin (five consecutive transfers in AM3).
The rate of increase was determined by growth at low phage/cell ratios (not exceeding 0.1 by the end of transfer) across five consecutive transfers, using phage titers measured from the end of the second flask to the end of the fifth [see 17 for details].
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Last year began with not one but two consecutive transfers to the West End – for the first time since 1968 – and ended with the news that the theatre's production of Jez Butterworth's play Jerusalem was on its way to Broadway (where it will open in April).
After two consecutive transfers, the microbial cultures were centrifuged (8000×g, 10 min, 4 °C) and washed twice with sterile 0.85% (w/v) NaCl.
However, the Thermotoga transformants lost their recombinant genes after three consecutive transfers.
However, the cellulase activities were lost in T. sp. strain RQ2 after three consecutive transfers.
The "asexual generation" (AG) is defined as the seven-day period separating two consecutive transfers.
After two consecutive transfers to fresh medium, this was used to inoculate the fermentation medium.
A. succinogenes was considered prototrophic for a vitamin, if growth was maintained for three consecutive transfers in its absence.
L-ascorbate at concentrations of up to 10 mM did not affect growth over at least nine consecutive transfers.
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