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After five consecutive passages in liquid culture with the CHA strain we obtained a bacteriophage stock that showed the same plating efficiency on both CHA and PAK strains.
Adaptation to new media was done through at least five consecutive passages, until a stable growth rate had been achieved.
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He's almost as able a narrator as Mr. Simpson, as on the opening of "It Ain't All Flowers," which flirts with ZZ Top-esque swamp-blues rock, or on "Long White Line," which opens with four different guitar approaches in four consecutive passages.
Analyses were carried out for six consecutive passages.
Recovered viruses were then subjected to two consecutive passages in RAW264.7 cells.
Engraftments of treated tumour tissues were performed for four consecutive passages.
Once increased titers in the presence of S20 were detected for two consecutive passages, the viruses were plaque purified.
Clones were picked individually and disaggregated cells were cultured for 3 weeks at clonogenic density for two consecutive passages.
Twelve clones for each antibody variant were expanded to T25 flasks, and their growth behavior and productivities were measured for ten consecutive passages.
Concentration of the MTX in the culture medium was increased by two-fold steps, each after two consecutive passages, until the cell viability decreased below 85%.
To select and amplify drug-resistant HSV in mice, a total of seven consecutive passages were performed, and progeny virus from each passage was characterized in vitro by PRA and PEA.
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CEO of Professional Science Editing for Scientists @ prosciediting.com