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Kinetic parameters were obtained by curve fitting of the data with KaleidaGraph ver 3.51.
The black dashed line shows the least-squares fitting of the data.
The kinetic parameters for oxamate at pH 7.0 were calculated by curve fitting of the data with GraFit version 7.0.3.
The fluorescence lifetime was estimated from a curve fitting of the data to a single exponential decay function.
We compute the first order polynomial (linear) least-squares fitting of the data in Fig. 4, and correlation coefficient is generally larger than 0.65.
Naiya et al. (2008) applied Dubinin Radushkevich isotherm for the curve fitting of the data obtained at the attainment of equilibrium.
In addition to the RMS values, fitting of the data clarified that the model is sensitive to the relevant anomalies and the conductor has a valid presence.
Following curve fitting of the data to the Boltzmann equation, the melting temperature (Tm) of each xylanase was calculated using Bio-Rad CFX Manager software (Bio-Rad).
However, fitting of the data to a specific binding model suggested that subtle differences in the diffusional behaviour existed between the materials.
A Shirley background was employed during fitting of the data (Shirley, 1972).
The solid lines in Figure 1 represent the nonlinear curve fitting of the data points based on eq 1.
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