Dose-response data were analyzed using the benchmark approach by fitting dose-response models to the various endpoints.
The fit at 32°C yields the same adaptation parameters as obtained from fitting dose-response curves of adapting cells [ 47].
The results of our analysis support the theory of non-sequential cooperative access of Ca2+ to CaM binding sites, and also allow the derivation of cooperatively effected dissociation constants, thus providing a more realistic tool for fitting experimental dose-response curves.
The fitting of their dose response model appears to be very stable with α/ β ranging from 8 Gy to 14 Gy.
Dose fitting curves were generated using GraphPad Prism 6 with the equation y = 100[1 – x m /(n m + x m )], where y is the percentage of inhibition, x is the siRNA concentration, n is the IC50 value, and m is the Hill coefficient.
Cumulative activities were estimated using mono-exponential fitting, and organ doses were estimated using OLINDA/EXM.
Stimulatory concentration (EC50) values were obtained by non-linear regression analysis using sigmoidal fitting with a dose-response curve by means of a scientific graphing software (SigmaStat, Jandel Scientific).
For the CO scans, CO was inhaled through a loose fitting mask at a dose of 3 MBq ml−1 and at a rate of 500 ml min−1 for 6 min.
This study presents MCDoseE 2.0, a new fitting program for ESR dating dose response curve (DRC) fitting and dose calculation.
Results were analyzed using FlowJo 7 (Tree Star Inc., Ashland, OR, USA) for Windows, and pharmacological interpretation was done using a four-parameter logistic equation with iterative fitting (dose response fitting) using OriginPro 8 (OriginLab, Northampton, MA, USA).
