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This was achieved by fitting a term for genotype (0,1,2) × sex (0,1) to the regression models.
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This method compares physical maps of genomes with genetic maps by fitting a 4-5 term polynomial functions.
The trend across quintile was assessed by fitting a linear term for exposure quintile.
We also formally tested for interaction between maternal age and admission by fitting an interaction term between them and conducting a likelihood ratio test at the 5% level of significance.
Mortality amongst HIV-negative individuals in the two earlier phases, and mortality among PLWH in each ART phase, were compared by fitting an interaction term in the model between HIV status and ART phase.
We evaluated the possible modifying effect of baseline vasomotor symptoms on effects of treatment at one year in health related quality of life by fitting an interaction term(s).
Finally, we tested for a difference in these three slopes (of the linear estimates for APOE genotype by smoking status: present, past, never smoking) by fitting an interaction term between APOE genotype status and smoking status in the logistic regression model and using likelihood ratio test to investigate whether a simpler model (without the interaction term fitted) better explained the data.
Extra analyses were performed to test the assumption of no carryover effect by fitting a carryover effect term into the model.
For baseline characteristics expressed as continuous variables, least-squares means were used to present the age-adjusted means, and tests for trend across blood pressure groups were computed by fitting a linear trend term.
To fit a quadratic term as a fixed effect, we z-transformed the variable and included all terms (linear and quadratic) in the model [ 48].
In the model we controlled for long-term trend and seasonality by fitting a non-parametric spline term for time (in days) to the outcome time series.
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