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Reactions are carried out in screwcap vials fitted with Teflon cap liners.
was fitted with Teflon filters to measure the gravimetric mass of five size fractions of the indoor PM: > 2.5 μm, 1.0 2.5 μm, 0.5 1.0 μm, 0.25 0.5 μm, and < 0.25 μm.
The remaining homogenate was sedimented at 40,000 × g for 15 min; the resultant pellet was then washed by resuspension (Polytron) in homogenization buffer followed by resedimentation, and was then dispersed with a homogenizer (smooth glass fitted with Teflon pestle) in 50 mM Tris buffer (pH 7.4).
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The ultrafiltration experiments were carried out in a batch type, dead end cell (UF cell-Amicon, USA) with an internal diameter of 76 mm, fitted with Teflon-coated magnetic paddle.
Reduction experiments were conducted on a 0.5 L laboratory autoclave fitted with a Teflon reaction beaker and a double impeller configuration consisting of an upper axial impeller and lower Rushton turbine.
The top of the sparging flask was fitted with a Teflon cap that held several pieces of 0.010" inner diameter Tefzel tubing.
Semi-anaerobic fermentations were carried out in 500 ml Erlenmeyer flasks filled with 550 ml of culture media and fitted with a Teflon stopper pierced with a syringe needle for gas exchange.
Tissues were homogenised in ice-cold 0.5 N perchloric acid at the rate of 0.4 ml per 100 mg tissue wet weight using a Camlab 563C homogeniser (speed 8) fitted with a teflon pestle.
Measurements were made on a VG-PlasmaQuad-2 (VG-Elemental, Winsford, UK) inductively coupled plasma mass spectrometer (ICPMS) fitted with a Teflon V-groove nebulizer and Gilson 222 autosampler.
The membrane pellets were resuspended using a smooth glass homogenizer fitted with a Teflon pestle, in a buffer consisting of 250 mM sucrose, 2 mM magnesium chloride, and 50 mM Tris (pH 7.5).
The membrane pellet was resuspended (Polytron) in the original volume of buffer and resedimented, and the resultant pellet was resuspended using a smooth glass homogenizer fitted with a Teflon pestle, in 10 mM sodium potassium phosphate buffer (pH 7.4) containing 150 mM NaCl.
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