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First, the Cox model was fitted with stage alone or with stage and the linear protein combination, and survival was predicted.
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The optical setup was built around a Nikon TE2000-U inverted microscope fitted with a stage riser for the fitting of two stacked carousels.
A sample holder was fitted with 3D stages so that different samples could be analysed without the need for opening the chamber in between sample analyses.
The set-up consists of an inverted optical microscope, fitted with an adapted stage on which two micromanipulators are employed to hold a pair of micropipettes.
Micrographs were obtained using a Zeiss microscope fitted with a heated stage and a Hamamatsu Orca CCD camera.
LNA-FISH signals were visualised on a Zeiss 510 Meta confocal microscope equipped with a Plan-Apochromat 63×/1.4OilDIC lens, fitted with a motorised stage.
Both microscopes were fitted with a motorised stage and used AxioVision software (Carl Zeiss).
Slides were examined using a Zeiss LSM Meta-confocal microscope fitted with a motorized stage.
Stained embryos were imaged in Multiscreen plates using a Nikon AZ100 microscope fitted with an automated stage (Prior).
Briefly images were captured using a × 40 plan apo objective from a BH2 microscope (Olympus, Tokyo, Japan) fitted with an automatic stage (Prior Scientific Instruments Ltd., Cambridge, UK) using a video camera (HV-C20; Hitachi, Tokyo, Japan) and were analyzed with Image-Pro Plus 4.5.1 software with a Stereology 5.0 plug-in (Media Cybernetics, Wokingham, Berkshire, UK).
But throughout much of the form's history, proscenium stages have been fitted with "aprons" or "fore-stages" that carry the stage through the arch into the house and thus make it a variation on the end stage form.
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