Field parameters were measured using a portable multiprobe system (YSI 556 MPS) with pH/ORP, conductivity/temperature, and DO sensors fitted to a flow cell.
The results were fitted to a Randles type cell by replacing the Warburg impedance with a mass transfer resistance in parallel with a constant phase element.
The array, composed of 24 electrodes 5 μm wide, was mounted on a chip carrier and cleaned with 2-propanol, ethanol, and deionized water before being fitted to a home-designed electrochemical cell.
The standard curve, which ranged from 5 to 2500 fmol per one million cells (fmol/106 cells), was fitted to a quadratic regression model weighted by 1/concentration.
For now, visitors are being invited to look inside the finished cockpit: a huge and complex monocoque (single piece shell) crafted from multiple layers of carbon fibre to produce what the designers believe is the strongest safety cell ever fitted to a racing car.
After curcuminoid quantitation, data points were combined from three independent experiments, normalized to 106 cells, and fitted to a ligand binding function (y = (BMAX*x / KD+x)+NSx) or a hyperbolic decay function (y = ab/(b+x)) using SigmaPlot™(11.2) as described elsewhere [ 36].
Average trace (black ± SEM.; n=6 cells) was fitted to an exponential function (red) to retrieve time constants of EGFR fluorescence intensity lossat the RE and its gain at the PM.
The resulting damaged cell fraction vs. radiant exposure data were fit to a logistic distribution and the cell damage threshold was taken from the fit as the radiant exposure causing a 50% of the irradiated cells to be stained [ 26].
The incline is the angle between the section radius through the center of an ellipse fit to a cell and the major axis of that ellipse extended towards the x axis.
Data (n = 8 cells) were fit to a Boltzmann Sigmoidal function (solid red line) with V50 = 254.3 nM (black dashed line).
