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The four Srb subunits that belong to the Head module could be deduced from the fitted crystal structure, and the only Srb subunit (Med21/Srb7) of the Middle module was clearly localized by nanogold labeling.
We also deposited an XML file of the FSC plot between the dynein alone map and the fitted crystal structure of the motor domain (PDB ID: 4AKG [ Schmidt et al., 2012]), as a supplementary file to the dynein alone submission EMDB-60133).
Calculation of FSC curves between the cryo-EM maps and rigid-body fitted crystal structures for the 60S and 40S subunits (Ben-Shem et al., 2011) confirmed the estimated resolutions, as well as the variation in quality of the density across the maps.
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The two remaining extra unoccupied volumes, both present above the N-terminus of each fitted RrgBD2 D4 crystal fragment (Figure 6A), easily accommodated the volume of two computer modelled D1 domains (RrgBD1) (Text S4, Figure S3A), each containing 156 residues.
We fitted the crystal structure of the Thermus thermophilus SmpB into our E. coli SmpB density.
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We have attempted to fit crystal structures of archaeal Orc1/Cdc6 into our EM volume.
(B ) A difference map (blue mesh) was calculated between a model of the CtpS filament calculated from the fit crystal structure and the EM structure.
Ghose et al. [22] in fitting their crystal truncation rod (CTR) data postulated four possible surface cleavages for this surface.
The indexing fits single crystal data as well as the values of volumic mass, ρexp=7.04(4) g cm−3 for ρX=7.096 g cm−3 for Z=4.
The critical resolved shear stresses (CRSSs) were determined via an inverse process of fitting a crystal plasticity finite element model to the experimental load displacement data.
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CEO of Professional Science Editing for Scientists @ prosciediting.com