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Specifically, there were 189 chemical assay pair special cases, which can be seen from the curve fits provided in the Supporting Information.
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Concentrations of analyte in samples were determined by interpolation from 20 individual standard curves, using the 5 parameter curve fitting provided in Bio-Plex Manager™ software version 3.0.
Curve fitting is performed using the five-parameter logistic fit provided in SoftMax Pro v5.2 (Molecular Devices, Sunnyvale, CA).
Given the statistical measures of model fit provided in Tables 2 and 3 (adjusted R, AIC, and root MSE), all three models show adequate and substantially similar model fit.
There is a fit in 2c, but no fits are provided in 3b to evaluate whether the data are truly exponential.
NMRD data and fits are provided in the Supporting Information.
Parametric results of the model fits are provided in Table 1.
In order to examine the fit of each item to measure a unidimensional construct (e.g., PWB), the infit and outfit mean square (MNSQ) item fit statistics provided in the WINSTEPS program were evaluated.
The overall FOI (λa) and domestic transmission rates (βdom,a) were estimated separately for each age-group (Table 1) by visually fitting the model to the age distribution of notified cases (national data from OSIRIS; data and model fit are provided in the Additional file 1: Figure A2).
R 2 values from the fit are provided in the text.
The data used for the fit are provided in Table 2.
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