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We have used the ratio of errors of exponential to piecewise linear fits of the initial portion of the AP in phase plot representation as a measure of the AP initiation dynamics.
Michaelis Menten parameters were determined from hyperbolic fits of the initial rates as a function of DNA substrate concentration.
The red curves in Figure 2A show fits of the initial kinetic profiles to eq 3, with the resulting kinetic parameters summarized in Table 1 and Figure 3A.
βd is given by 7 The green curves in Figure 2A show fits of the initial dissociation profiles to eq 6, with resulting parameters summarized in Table 1 and Figure 3B.
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The returns data prior to July 1993 are used for the initial fit of the initial market distribution.
The nuclear import rate of Ci-GFP was calculated as the slope of a linear regression fit of the initial phase of the flux curve (at least 5 post-bleach points).
As the model fit of the initial CFA model was not sufficient according to the CFI and TLI indexes (see Table 2), we tried to improve model fit by dropping items showing large modification indexes for cross-loadings and ambiguous item wordings.
These results suggest that the fit of the initial three models needs improvement, both in relation to variance in the population as well as in relation to the independence model.
The fit of the initial parts of the titration curves ([CBZ] ≤ 1000 μM) with the Hill equation is consistent with positive cooperativity, with a Hill coefficient (h) of 1.4 ± 0.4 (S50 = 162 ± 4 μM, kcat = 1.9 ± 0.3 min–1); this is in agreement with a previous report in which CYP3A4-dependent epoxidation of CBZ was characterized as exhibiting inherent homotropic cooperativity with h = 1.4 1.7.
Values of kcat and Km for the LDM cTIM-catalyzed isomerization of GAP (0.075 11 mM) in solutions that contain 30 mM TEA buffer (pH 7.5) at I = 0.1 (NaCl) and 25 °C were determined from the nonlinear least-squares fit of the initial velocity data to the Michaelis Menten equation.
The kinetic constants were determined from a fit of the initial velocity data to eq 1 using SigmaPlot 9, where v is the initial velocity, Et is the total enzyme concentration, kcat is the turnover number, [A] is the substrate concentration, and Km is the Michaelis constant.
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