Pan, J. et al. Interrogation of mammalian protein complex structure, function, and membership using genome-scale fitness screens.
A user would set up the system's software by filling out a profile on what Mr. Kriger calls the "fitness screen".
We additionally performed context-specific fitness screens for resistance to or synergy with trametinib, a Ras/ERK/ETS inhibitor, or rapamycin, an mTOR inhibitor, and identified key regulators of each pathway.
Under basal growth conditions, 1235 genes were essential for cell fitness at a false-discovery rate of 5%... We additionally performed context-specific fitness screens for resistance to or synergy with trametinib, a Ras/ERK/ETS inhibitor, or rapamycin, an mTOR inhibitor, and identified key regulators of each pathway.
In the case of a highly studied organism like the budding yeast, S. cerevisiae, microarray expression data for the entire genome under a variety of perturbations is available [13], and several global rapamycin fitness screens have been carried out [14] [17].
Drug sensitivity data were obtained from the heterozygous fitness screen of Hillenmeyer et al. (2008).
These include experimental limitations, such as the poor sensitivity of fitness screens (32) and the narrow range of cultivation condition tested (generally complex medium) (33).
In C. albicans a fitness screen of nearly 3000 heterozygous mutants lacking one copy of each gene, termed a haploinsufficiency screen, was used to investigate the mode of action of novel compounds with potential antifungal activity (Xu et al., 2007).
In the case of the SEA complex, the link has been inferred from genome-wide pairwise fitness screen data (Beck et al. 1999; Dokudovskaya et al. 2011; Schmidt et al. 1998).
The correlates of obesity in Chinese children are similar to those reported in studies conducted in other countries, including family background (family income, parent education, parent weight status), birth weight, breastfeeding, physical activity, physical fitness, screen time, diet, access to physical activity and healthy foods, and regions of residence [ 28, 29, 40- 42].
