Each plot shows the average fitness of the methods described in Section 2.4 (solid red line) and Section 2.3.2 (dotted blue line) relative to the simplest, baseline described in Section 2.2, and its variation as a function of the number of generated hypotheses.
To demonstrate the fitness of the proposed method for applications for the purpose of ligand-based virtual screening, the enrichment performance was tested against datasets from the DUD-E [10].
We calculated a demographic measure of relative fitness (w) by the method of Charlesworth (1994), following Peters et al. (2003).
Unless the mutations of interest alter the relationship between what is measured and the true value of fitness, the specifics of the method employed are unlikely to affect the shape of the inferred DFE from any given experiment.
In laboratory-generated cross-feeding bacterial consortia, NFDS is estimated to cause a selection coefficient of up to 0.14 [ 52] (calculated from the reported relative Malthusian fitness, following the method of [ 53]).
The fitness-for-purpose of the method was demonstrated through successful validation at relevant environmental concentrations, i.e. the low nano- and picogram range.
As hypothesized, all simulations demonstrated an increase in average population fitness and percentage of DOs achieving maximum fitness regardless of the selection methods used (truncated selection or fitness-proportionate selection) and the complexity of various fitness requirements within 200 generations.
Fitness of selected method (Technology Mapping) was attested by experts who were involved in the process of identification; although the validity and reliability of proposed model rest to be tested by using it in other cases in different contexts.
A three-layered feed-forward artificial neural network (ANN) model with back-propagation learning algorithm was employed to model the nonlinear relationship between the spectral parameters and fitness of each regression method.
The relative fitness of the founder strain, determined by ξ (Methods), remains unknown.
As measured in short-term competition assays (see Methods), the fitness of the haploid mutator strain (YPS3460) relative to that of the wild-type strain (YPS3343) was 0.976 ± 0.002 (S.E.M) before propagation, which is significantly different from one (t 9 = 5.84, two-tailed p < 0.01).
