Akaike Information Criteria (AIC) for goodness-of-fit determination was used to estimate the optimal fit [36], [37].
Goodness-of-fit determination was acceptable for all, and the linear regression analysis R2 value was 0.954, 0.448 and 0.947 (P = 0.015; P = 0.088; P = 0.001) for ATN-ISS, PICARD and IRA-PUC, respectively.
The used evidences include high F-value (143.12), very low P-value (<0.0001), non-significant lack of fit, the determination coefficient (R2 = 0.99) and the adequate precision (47.067).
However, those methods are based on the amplitude measured on waveforms of intermediate period, or the amplitude of the initial parts after the onsets, and do not fit magnitude determination for great and long source duration earthquakes.
High F-value (10.01), very low P-value (<0.0001), non-significant lack of fit, the determination coefficient (R2 = 0.903) and the adequate precision (10.82) demonstrate good correlation between experimental and predicted values of the response.
As observed, the ANOVA of the model indicated that high model F-value, the values of Prob.>.F, the Lack of Fit, the determination coefficient, the RAdj2, the RPred2 and the adequate precision were 845.09, 0.0001 (<.0.0500), not significant, R2.=.0.999, 0.998, 0.994 and 95.94, respectively.
Real-time PCR reactions of the standard, test cDNA samples and no template controls (NTC) using the same primer set were analyzed together in the same 96-deep well plate (Bio-Rad) in order to minimize run-to-run variations and use exactly the same threshold setting (user defined baseline subtracted curve fit) for determination of the threshold cycle values (Ct).
Statistical fit and determination of Kd values were done using the IgorPro (Wavemetrics, Portland, USA) software.
This spectral fit enables determination of the hemoglobin compounds, resulting in estimation of oxygen saturation and blood volume fraction.
