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These scores were determined by the availability and accessibility of seaport facilities Fisheries MCS Illegal fishing score University of British Columbia (UBC) (Pitcher et al. 2006 c Each country was scored on whether vessels were fishing illegally in its fisheries, with '0' = no; '2.5' = occasionally; '5' = often'7.5.5' = a great deal; and '10' = almost as much, or more than legal vessels.
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Box plots of FISH score versus best overall response for each model were also constructed.
For each evaluable tumor, a FISH score was determined according to one of the five different enrichment models (Table 1).
An IHC score of 3+ or HER2 amplification by fluorescence in situ hybridization (FISH) score was used to determine HER2 positive status.
The process was repeated for each model for the analysis of PFS time versus the respective FISH score for patients in both study arms, with similar results.
The signal from the centromere probe functions as an internal control; the final FISH score is based on a ratio of the signals from the two probes.
HER2 was considered positive if HercepTest (Dako North America, Inc., Carpinteria, CA, USA) was 3+ or if the fluorescence in situ hybridisation (FISH) score was >2.0.
These plots did not demonstrate a visible correlation between EGFR FISH score and survival time for any model, in either study arm.
In an initial exploration of the predictive potential of EGFR FISH status, scatter plots were constructed for each model for survival time versus the respective FISH score for patients in both study arms.
ER and PR were considered positive if immune-histochemistry (IHC) staining was ≥10%; an IHC score of 3+ or HER2 amplification by fluorescence-in-situ-hybridization (FISH) score was used to determine HER2 positive status [ 24].
As expected, the median FISH score in models A, C and E which used more stringent criteria for defining a FISH-positive cell was markedly lower than in models B and D, which used less stringent criteria (supplemental Figure 1, available at Annals of Oncology online).
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