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"There are two sorts of islanders – those from fishing backgrounds and those from farming backgrounds.
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I come from a fishing background, and the novel struck a deep chord within me.
Importantly, we added data showing that human Dystrophin fully rescues the dystrophic phenotype indicating that huDys-GFP is functional in the fish background.
ImageJ software was used to quantitate mean relative fluorescence in an ROI in the larvae trunk above the posterior intestine (trunk ROI) and an ROI outside of the fish (background ROI).
Therefore, detecting real fish by background subtraction using a static model or using the most recent frames as the background model is not effective.
In M-FISH images, background usually contains most pixels, but the chromosomal regions are of most interests.
Two elements are key to standardizing MO injection: (i) the fishes' genetic background, and (ii) the MO dose.
We also re-evaluated the methods used to determine DAPT (positive control) performance and found an error: the use of non-transgenic fish as background rather than DMSO treated transgenic controls.
Fertilized eggs from wild type embryos (AB strain) and Tg[fli:eGfp] transgenic fish (TU/AB background, provided by the Lawson Lab, UMass-Medical Scollectedre collected, washed in embryo medium, and incubated at 28.5°C.
And in both the Bruegel and El Greco, if you look very carefully, there are tiny sticklike figures in the background (fishing in El Greco and playing on a village green in Bruegel): a level of detail barely visible but suggesting a Hemingway-like precision and desire to get things right.
In response to different backgrounds, fish altered their pigmentation through morphological color change (Fig. 1), which was consistent with a previous study on the same species (Hornsby et al., 2013).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com