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The samples taken from affected fish were positive for ISAV.
At 2 wpi, 5 out of 6 fish were positive for viral RNA in heart (median of relative copy number = 20.5 fold, Figure 2a).
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The selection differentials on reproductive investment imposed by fishing were positive (indicating that fishing selected for higher reproductive investment) but could be considerably lowered with simple management measures such as increasing the minimum size limit.
Of the fish species that were sampled from fish ponds, 87% were positive for V. cholerae.
Although all fish species tested were positive for brevetoxin (striped mullet, pinfish, mangrove snapper, sheepshead) (range: 3->20.3 ng/g), the striped mullet also concurrently contained DA (mean ± SE: 54±4 ng/g; n = 3).
For equivocal stating, silver in situ hybridization (SISH) or fluorescence in situ hybridization (FISH) were performed; the results were positive for HER2 amplification when the ratio of HER2 to CEP17 was > 2.2.
In the Sarasota Bay region, all fish collected in 2008 (n = 5 individuals of 5 species) were positive for DA.
FISH results were available for 18 of the 55 patients; the results were positive for all but two.
According to the FISH scoring guidelines, these cases are positive for HER2 gene amplification.
One of three fish samples submitted by probable case-patients was positive for ciguatoxins.
In contrast, only one of the 44 fish species (2.3%) sampled in the Mediterranean Sea was positive for V. cholerae (Tables 1 and S1).
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