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Six fish were anesthetized with MS 222 (tricaine methanesulfonate) before sacrifice.
Before sampling, all fish were anesthetized with 0.05 % 2-phenoxyethanol (Wako), and total length, body weight, and body colors were determined.
In accordance with the requirements of the German Animal Protection Law, all fish were anesthetized with an overdose of tricaine (MS-222) solution and subsequently killed by cutting the spine above the operculum.
Fish were anesthetized with 0.02% Tricaine (MS-222) and transferred to a moist sponge for surgery.
For analyzing GFP fluorescent pattern, embryos, larvae and adult fish were anesthetized with Tricaine (Sigma, Cat.# A-5040).
Fish were anesthetized with tricaine methanesulphonate (MS-222, Argent Chemicals, Redmond, WA), and total length (mm) and wet weight (g) measurements were taken.
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Fishes were anesthetized with MS-222 (Tricaine methane sulfonate 200 mg/L river water) at the site of capture.
Fishes were anesthetized with the addition of phenoxybarbtal in the water tank (3ml per 10 liter) and then sacrificed following the rules established by the ethical local committee.
Adult fish were anesthetized and injected with substances intraperitoneally at a volume of 25 μl.
For the final weighing, fish were anesthetized one at a time with 0.1 mg/L MS-222.
Following each trial, the fish were anesthetized, measured and, upon recovery, released with the non-experimental hatchery coho.
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