ALT status was determined by telomere FISH using the Telomere PNA FISH Kit/Cy3 (Dako, Burlington, ON, CA) following a generalized protocol as previously described [ 37].
In those cases with HER2, further analysis was with FISH using the DAKO K 5331, HER FISH PharmDxTM kit and hybridized in the DAKO HYBRIDIZER.
They then randomly repaired the fish, using the boldness scores to classify each fish as either "bold" or "shy," relative to its new partner.
We detected 51 vertebrate species, including 9 fish, using the 8 culverts after the construction of the barrier wall-culvert system, compared with 28 vertebrate species in the 4 existing culverts prior to construction.
Each fish was injected intraperitoneally with 0.1 mL of fresh bacterial suspension at a concentration of 2 × 108 colony-forming units (CFU /mL based on a preliminary virulence test (50% lethal dose, LD50) in groups of fish using the pathogenic bacterium E. tarda at different concentrations (106 − 1010 CFU/mL) for 7 days (data not shown).
Depending on the Solicitor General's decision on behalf of this Administration, non-bank entities will either have to fish using the catch-and-release method, or they'll get to use dynamite.
As assessed by FISH using the human-specific COT1 probe, CHO cells possessed an independently segregating mini-chromosome corresponding to the MI-HAC vector (Fig. 4B).
We targeted the XIST expressing chromosome by DNA/RNA FISH using the following probes: genomic single locus RP11-349A16 (Xq22.3), X painting and XIST.
We also show the cardiac hypertrophy process can be recapitulated in wild-type fish using the anemia-inducing drug phenylhydrazine (PHZ).
The HPV types 6, 16, 18, and 31 were visualized in sperm by fluorescence in situ hybridization (FISH) using the above-mentioned ZytoFast HPV-Typing Kit (ZytoVision, Bremerhaven, Germany) and a PE-conjugated streptavidin (BD Biosciences Pharmingen, San Diego, CA).
FISH using the 688 bp fragment as probe indicated that, in addition to the Y chromosome, similar sequences are located at the centromeric regions of all the autosomes and on the long arm of the X-chromosome (Fig. 5D).
