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However, species with large genomes and many repeated sequences, e.g., maize, may require SC spreads, single copy probes, more sensitive FISH techniques, and/or more effective means of blocking repeated sequences to effectively use BAC-FISH for checking genome assembly (Zhong et al. 2001; Stack and Anderson 2002; Koumbaris and Bass 2003; Kato et al. 2006; Wang et al. 2006a).
These probes were successfully tested by applying the FISH techniques on preserved cells of different clones of both species.
We have evaluated the use of PCR and fluorescent in situ hybridization (FISH) techniques for the detection of thermotolerant campylobacters in naturally contaminated chicken products.
In the last few years, fluorescence in situ hybridization (FISH) techniques have become a suitable and reliable tool for identification and differentiation of closely related species of protozoa, such as ciliates.
Denaturing gradient gel electrophoresis (DGGE) and fluorescence in situ hybridization (FISH) techniques were used to uncover the changes in the bacterial community of the biofilter during the deodorization processes.
Further emerging developments focus on sorting FISH-identified cells for subsequent single-cell genomics and on the direct detection of specific genes within single microbial cells by advanced FISH techniques employing various strategies for massive signal amplification.
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Tyramide signal amplification FISH (Tyr-FISH) technique has been successfully used for the identification of low copy number DNA sequences in wheat [ 47].
Fluorescence in situ hybridization (FISH), technique that employs fluorescent probes for the detection of specific deoxyribonucleic acid (DNA) sequences in chromosomes.
Aberrations were analyzed using the fluorescence in situ hybridization (FISH) technique with three different colored chromosome-painting probes.
Fluorescence in situ hybridization (FISH) technique was employed to characterize the nitrifying bacteria of the granular sludge.
Here we describe the application of the smRNA FISH technique to samples of Caenorhabditis elegans, a well-characterized model organism.
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