A positive FISH signal was defined as fluorescence in excess of that detected with the negative control FISH probes.
Briefly, at least 30 different tumor cell nuclei were analyzed for the FISH signal numbers per tissue spot.
m Haploid nucleus of a basidiospore showing a FISH signal.
A chromosome set had one FISH signal only as demonstrated in uninucleate basidiospores (Fig. 4m).
While single FISH signal was normally detected in postmeiotic nuclei at hyphal tips (Fig. 4v), nuclei with two FISH signals (Fig. 4x) or more were occasionally found.
Nuclei in Fig. 4v x were haploid resulting from meiotic-like division because there was only one FISH signal detected in each of the nuclei.
Moreover, in 50% of the experiments, no specific FISH signal was detected.
Using the ROI manager, the x, y, and z coordinates of each ROI (each FISH signal) are measured in µm.
Visualization of FISH signal was done with a fluorescence microscope (IX71 Olympus) and/or a confocal microscope (Olympus Fluoview).
The decline in FISH signal showed a slower rate of decrease compared to EGF reaching basal levels in ∼2 3 hours.
One FISH signal per interphase nucleus for autosomal DNA probes was detected in 6 12% of nuclei scored in different brain tissue samples.
