Following staining and neuromast quantification, fish were returned to 0.016% MS-222 and killed.
FISH and sry-quantification revealed improved survival of ShhMSCs post engraftment.
In addition, to the FISH-based telomere quantification methods, the telomere quantitative PCR (qPCR) is quite widely used as it does not require live cells and its fast and highthroughput [ 28].
Each biopsy was split into three parts: one for basic histopathological characterization, one for DNA extraction followed by measurement of ultra-short telomeres using Universal STELA, and one for measurement of mean telomere length by Q-FISH and for quantification of senescence through SAHF.
The combination of sentinel fish exposure with the quantification of waterborne stages allowed us to attribute this pattern in C. puntazzi density to higher numbers of actinospores in the water, while myxospores are predominant in summer and winter.
Since quantification of FISH signals have been applied, it was possible to differ between true hypodiploid (monosomic) and euploid cells featured by associated FISH signals (a signal appearance similar to monosomy).
(D ) Quantification of FISH signal in the first telophase bridge in male and female PEL embryos.
Holá et al. and Flem et al. both developed LA-ICP-MS methods for trace element quantification in fish scales as an alternative to otolith sampling [ 63, 64].
In addition, copy number differences for many of the CNVs listed in the above database are not confirmed using secondary independent quantification methods (FISH or quantitative DNA methods).
Quantification of FISH signal showed that the amount of poly(A)+ mRNA present in dFMRP granules that are induced by either ΔKH or ΔRGG (∼70%) is similar to that induced by GFP-dFMRP (Fig. 3B).
