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The AR FISH map for this TURP specimen is shown in Figure 4C.
The RH map and associated FISH map provide a valuable gene-ordered resource for gene mapping and QTL studies.
To compare our pachytene FISH map directly to the genetic linkage and draft genome assembly maps, we used a standardized map unit relative map position (RMP) to produce the comparative map alignments.
Unlike a FISH map that is not altered or modified by the addition of a new dataset, an RH map does change after adding information because different algorithms provide different solutions for local marker order.
In comparing the pachytene FISH map to the genetic linkage map [ 7], we found that the linear order of markers in the linkage groups was in complete agreement with the order of the corresponding fosmid clones along the chromosomes except for 2 10 and 2 11.
To compare our pachytene FISH map directly to the genetic linkage map and draft genome assembly map, we used a standardized map-unit system in which the percentage distance of each locus along the chromosome in each map was used, denoted as RMP units.
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Robledo, G. & Seijo, G. Species relationships among the wild B genome of Arachis species (section Arachis) based on FISH mapping of rDNA loci and heterochromatin detection: a new proposal for genome arrangement.
In addition, results from FISH mapping of ribosomal rRNA genes indicates that the duplication of 25S rDNA loci occurred independently in subgenus Parvisepalum and the sections Coryopedilum and Pardalopetalum of subgenus Paphiopedilum, while the duplication of 5S rDNA loci can be detected only in subgenus Paphiopedilum (Lee and Chung 2008; Lan and Albert 2011).
We also report interphase fluorescent in-situ hybridization (FISH) mapping of two de novo apparently balanced chromosomal rearrangements associated with BRAHD and determine the embryonic kidney expression of genes positioned close to the breakpoints.
We also compared the FISH mapping of telomere and sGISH.
The 266 FISH mapped contigs were evenly distributed and covered 60% of the genome (95,668 kb).
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