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We thank M. Rath and J. Gonzales for help with fish maintenance, and S.-Y.
Overall, the data show that the application of biofloc technology for tilapia brood fish maintenance and larval production can improve Nile tilapia fry quality and production performance.
The authors thank Dr Daniel Garcia de la serrana Castillo and Dr Daniel Macqueen for critically reading the manuscript and Mr Luke Bower for help with fish maintenance.
We are very grateful to Lauren Parker and Phil Evans and ESRF staff for help with data collection, to Hiba Codore for guidance and support with fish maintenance, to Jake Thieman for imaging advice, to Vimlesh Kumar for the anti-Syndapin serum and to Alexander Schier and Lilianna Solnica-Krezel for plasmids.
MAC, CV and HT were involved in fish maintenance, feeding trial and sampling.
Fish maintenance, staging and husbandry were as described previously (Alibaud et al., 2011) under standard conditions (Westerfield, 2007).
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For aquatic vertebrates like fish, the maintenance of osmotic and ionic gradients between body fluids and the external environment for internal homeostasis are more challenging.
Always unplug all your filters and heaters before preforming this type of fish tank maintenance.
After the fish tank maintenance and the aquarium water change is complete, drain the sink with the aquarium decorations in it, dry them off and allow them to cool to room temperature.
Remove any organic aquarium decoration (driftwood) from the sink and with only the inorganic aquarium decoration left, fill the sink with hot water and allow the aquarium decorations to soak while you perform the rest of your routine fish tank maintenance.
Declining cash incomes and dwindling international aid is impairing the ability of producers to acquire seeds, fertilizer, spare parts for greenhouses, irrigation facilities and fishing boat maintenance.
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