Serum cortisol levels were measured in fish sampled in 2008 and 2009, using a commercially available cortisol enzyme linked immunosorbent assay (ELISA Neogen Corporationn, Lexington, KY), that has been validated for use in salmonids [26].
TNF-alpha, interleukin-6 (IL-6) enzyme linked immunosorbent assays (ELISA's) were purchased from Research and Development Systems (Abingdon, U.K.).
Commercially available enzyme linked immunosorbent assays were employed.
Enzyme linked receptor denotes enzyme linked receptor signaling pathway.
GI: Gastrointestinal ELISA: Enzyme linked immunoabsorbent assay;F.
NGAL was measured by the enzyme linked immunosorbent assay (ELISA).
Chemokine concentrations were determined by enzyme linked immunosorbent assay.
ELISA, enzyme linked immunosorbent assay; FACS, fluorescence activated cell sorting.
A membrane-bound enzyme linking lipid a and ptdins-4-p biosynthesis.
IL6 and IL10 were determined by Enzyme Linked Immunosorbent Assay.
Immunogenicity was evaluated by Enzyme Linked Immunospot (ELISpot), flow cytometry and Enzyme Linked Immunosorbent assay (ELISA).
