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After the patient complained of the abnormality, the authors firstly inserted the PAC to evaluate the cardiac function.
The needle was firstly inserted perpendicular to the tendon, with a second pass aimed 20° superiorly in the plane of the tendon, and a third pass aimed 20° inferiorly.
The 938 and 992 bp BoHV-4IE1 left and right arm amplicons were firstly inserted in pJET1.2, a commercial vector from Thermoscientific, designed to directly subclone blunt-ended PCR amplicons (as those generated by Pfu polymerase amplification), using the CloneJet PCR Cloning Kit (Thermoscientific), generating pJET/IE1left and pJET/IE1 right.
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Firstly, insert a link or browse any picture to upload.
Firstly, inserts were excised from each of the constructs in pBluescript II KS+ phagemid using Xba I/Kpn I restriction enzymes and cloned into the pShuttle expression vector.
Firstly, an inserted transgene may disrupt native genes.
Firstly, we inserted a six amino acids TC tag upstream and/or downstream of each of the protease cleavage sites in the Gag or Gag-Pol precursor protein.
When a key value is written into LevelDB, it is saved into commit log file firstly, then it is inserted into a sorted structure called MemTable.
We started the work with locating the oxygen binding site of AlkB, whereby we attempted to model dioxygen into the protein structure at various metal binding sites, that is, create structures A and B. Firstly, molecular oxygen was inserted into the sixth binding site trans to His131 by replacing the water ligand.
Firstly we report the elements inserted in the same genome context in different strains, indicating preferred sites of insertion; that, the ones related to common ancestral events.
All GITR transmembrane-cytoplasmic mutants have been firstly cloned by inserting PCR mutant products into the pCR3.1 plasmid (Invitrogen), then cut by NotI and NsiI restriction enzymes and subcloned into NotI and NsiI sites of GITR full length.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com