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The analysis revealed that the designers produced an initial "first-pass" solution to the given design brief in a breadth-first manner, with this solution addressing several easy-to-handle requirements.
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These solutions are by no means exhaustive, but they represent "first-pass" guidelines that organizations can set up and build upon.
Fresh crystals were first passed stepwise through cryoprotectant solutions as described previously and then soaked with 10 mM inhibitor for 4 6 h at 4 °C before being flash-cooled with liquid nitrogen.
After that, the sections were air-dried at room temperature (RT) for 30 min and thereafter fixed in 4% paraformaldehyde (PFA) in 0.1 M phosphate buffer (diluted in DEPC-H2O) (the PFA solution was first passed through a 0.45 μm sterile filter) for 1 h at RT.
Moreover, we gave the solution through interperitioneal injection, reducing the first pass effect.
This strategy is therefore likely to remain a feasible solution to explore microbial evolution in a first pass step, utilizing all of the ever increasing genomic data [ 35].
Once built during the first pass over D, this DAG is then visited in pre-order to iteratively extract each such solution in turn.
The second pass (Supplementary Algorithm S1.0.1) is a traceback algorithm that reads information from each to construct an optimal solution.
Seconds passed.
A split second passed.
An awful second passed.
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CEO of Professional Science Editing for Scientists @ prosciediting.com