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To avoid uneven color, first wash with a soapy washcloth to remove dead cells that are ready to flake off.
An additional 6% was removed in the first wash with 30 mM imidazole.
The beads were washed three times: the first wash with RIPA buffer and the others with PBS.
Three mL of first wash with 50 mM MES (pH 5.6) were collected at a flow rate of 1 mL.min-1.
Following the incubation, the supernatant containing unbound anti-OTA Mabs was removed and the beads were washed three times with storage buffer (the first wash with 500 μL, the remaining two steps with 1 mL) consisting of PBS (5.4 mM Na2HPO4, 1.3 mM KH2PO4, 150 mM NaCl, 3 mM KCl, pH 7.4) to which, 0.02% Tween-20 and 0.05% NaN3 were added.
First wash with milk and then with water.
Similar(52)
However, when we performed this experiment on EGTA-washed immunoprecipitates that were first washed with TBS to remove the EGTA, addition of Ca2+/CaM had no significant effect on hVps34 activity.
Nix is applied to damp hair first washed with a nonconditioning shampoo.
The obtained solid was first washed with petroleum ether, followed by ethanol washing and vacuum drying.
Cells were first washed with PBS and detached with 0.2 mL of 0.25% trypsin.
The samples were first washed with seawater and the debris was removed manually.
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CEO of Professional Science Editing for Scientists @ prosciediting.com