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The first was kept out by Neil Sullivan's legs.
The first was kept as a control; to the second fraction was added proteinase K (0.1 mg/ml); and to the third fraction, we added both proteinase K as well as the detergent NP40 (at a final concentration of 1%).
The first was kept as a control (NT); proteinase K (0.1mg/ml) (Prot.K) was added to the second fraction; and to the third fraction, both proteinase K and the detergent NP40 (at a final concentration of 1%) were added (Prot.K/NP40).
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The first is kept at Borough Hall.
One third (n = 6) received a low-fat diet for 24 weeks, one third was kept on an extended high-fat diet (eHF), and the remaining was switched from low-fat to high-fat chow 3 weeks before sacrifice (sHF).
A gap of 120 seconds was kept between subsequent injections.
The remaining fifth was kept for subsequent qRT-PCR validation.
The section thickness was kept at 1mm, the reconstruction thickness was kept at 0.8mm.The gantry rotation time of 0.5 seconds was kept for all the three phases.
One aliquot was used for direct viral testing by the DFA and the second was kept at −70°C for nucleic acid extraction and virus isolation.
The top third of the 5th leaf was collected for DNA extraction, whereas the bottom third was collected for RNA extraction (the middle third was kept as back-up).
To select markers, an increasing proportion of SNP were sequentially removed solely considering their map position, so that for instance: to reduce a panel to 50%, every second marker was kept for analysis, for 25% every fourth was kept and so on.
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