For the first two crosses, DNA was extracted from leaf tissue collected from individual plants.
All black N2 males from the first two crosses described above were genotyped for 12 markers (D1Mit432, - 480, D5Mit353, - 311, D9Mit60, - 262, D11Mit5, - 67, D8Mit234, - 211 and D17Mit28 and - 142), two spanning each of the six QTL harboring regions (MMU1, 5, 8, 9, 11 and 17; Table 1 and Additional File 1).
Thirty-two of 32 zygotes and 31 of 32 zygotes were able to form colonies in the first two crosses (with single elg1 Δ and srs2 Δ mutants, respectively), whereas only 2 of the 80 elg1 / elg1 srs2 / srs2 zygotes formed viable colonies after micromanipulation.
The displacement distance at the first zero crossing of the EAP averaged across directions orthogonal to the primary fiber orientation in the corpus callosum is found to be larger in the body (5.65 ± 0.09 mum) than in the genu (5.55 ± 0.15 mum) and splenium (5.4 ± 0.15 mum) of the corpus callosum, which corresponds well to prior histological studies.
When the criteria are met, the MCS closes at 3.505 s, when the first zero crossing occurs.
OBS record sections were produced by 4- to 16-Hz bandpass filtering, predictive deconvolution (first zero crossing, operator length of 660 ms), and a local slant stack to enhance the signal-to-noise ratio.
The signal part with significant energy is determined by identifying the time interval with starting point as the first zero crossing prior to the peak, around 35 ns and end point as 60 ns after which the received energy is at the noise floor.
We have calculated them by integrating the ACF up to its first zero crossing.
To analyze the resulting simulation dynamics, we used the first zero crossing of the autocorrelation function.
The first zero crossing of the ACF (FZCA) occurs at a delay of 0.264×10 MC times steps.
