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For the second transformation (Fig 1, left side), the URA3 strain created in the first transformation was co-transformed with a high-copy LEU2 plasmid (YEp351) and a 600 900 bp PCR fragment spanning the URA3 insertion site.
In the second transformation, the strain created by the first transformation was co-transformed with a high-copy TRP1 plasmid (pTV3) and PCR fragments that span the URA3 insertion site.
As Ball explains, it was not Muybridge's first transformation.
Customer service Sponsored by Picker Institute Europe Birmingham city council for their Customer First transformation programme.
Information is given about the process and outcomes of this first transformation step in practice.
The conditions that brought about the first transformation of American society are strikingly similar to those we see today.
Only compositions with 2 ⩽ x < 4 form a metastable quasicrystalline phase during the first transformation step.
The first transformation was from amorphous to the monoclinic LaCrO4 monazite type compound.
The first transformation in communication technology was the alphabet and the second, the printing press, which dominated Western culture from the 15th to the 20th century.
Like the best technology companies, it has learnt the knack of re-invention.The trigger for Oracle's first transformation was an earlier market shock.
The first transformation implements averaging, and it improves peak detection accuracy but not speed; the second transformation implements coincidence detection, and it improves speed but not peak accuracy.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com