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Comparison of the sequence indicated that the changes arose during rescue or during the first three passages of the rescued viruses.
The current data based on the first three passages have already demonstrated a better genetic stability in MRC-5 cells than in Vero cells.
Monolayer cultures derived from all 12 primary isolates (6 normal, 4 clear cell RCC and 2 chromophobe RCC) and the corresponding first three passages were examined by light microscopy under an inverted optical microscope.
Cells were used in the first three passages.
For the first three passages 200 µg/ml Gentamicin (Gibco) was added to the media.
In all our experiments, only the first three passages of each HUVEC primary culture were used.
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For the first two passages, there were 10 tasks, five for each.
Cells of the first four passages were used for these studies.
All experiments involving calvarial osteoblasts were conducted within the first two passages from the time of isolation.
Human primary foreskin fibroblasts HS68 were purchased from ECACC and used at subconfluence within the first ten passages.
Allantoic fluid and the cell culture supernatants from the first five passages were tested for hemagglutinating activity with a 1% suspension of chicken erythrocytes to exclude avian hemagglutinating viruses (e.g., influenza virus and EDS-76 virus).
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