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Human primary foreskin fibroblasts HS68 were purchased from ECACC and used at subconfluence within the first ten passages.
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For the first five passages, the cells were plated at 500 cells/cm2 and incubated for 3 5 days.
For example, the first two passages above seem to relate memory to acts of speaking and reading.
Human neuroblastoma cell U251 cell line was procured from NCI and used for the experiments within the first five passages after revival.
We observed a significant decrease in the rate of growth (Fig. 1d) and increased cell death (Fig. 1e) in these tumors over the first three passages.
He was hit, he later wrote "by that sweetly ecstatic, rare experience that I have heard described as a 'moment of clarity'". This being the CIA, the solution was kept a secret, but it was solved separately by Jim Gillogly, a California computer scientist, who published the first three passages in 1999.
For the first two passages, there were 10 tasks, five for each.
The first four passages are all neumed in other manuscripts, such as Munich, Bayerische Staatsbibliothek, clm 21562, so these episodes are prominent in the medieval reception of Virgil more broadly.
Cells of the first four passages were used for these studies.
All experiments involving calvarial osteoblasts were conducted within the first two passages from the time of isolation.
The current data based on the first three passages have already demonstrated a better genetic stability in MRC-5 cells than in Vero cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com