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Strips were first stained for total protein detection with Ponceau red, and the images of the stained dots were captured by scanning.
The macromolecules, such as lipids, proteins and peptides present in the fingerprint residue are first stained with colloidal gold and then by a silver developer.
The gel was first stained with Fast Red dye for esterase activity against 1-naphthyl acetate (purple bands) followed by staining with Coomassie Brilliant Blue (blue bands).
After the cover-slip was removed, the slides were first stained in Myers modified hematoxylin solution (#S216-16 oz; Poly Scientific R&D Corp) for 1 min and then washed with tap water.
MCA segments were first stained with hematoxylin (HTX) and inspected for general morphology; this revealed a thin adventitia, a smooth muscle cell layer, an internal elastic membrane and a single layer of endothelial cells.
We first stained vinculin, a component of focal adhesions, in order to visualize these focal adhesions.
Slides were first stained by IF and fluorescence signals (green color) were captured using a FITC filter.
24 hours later, renal graft-infiltrating cells were isolated and first stained with anti-CD4-PE or anti-CD8-PE.
Cells on the filter were first stained with Liu's stain and cells that remained on the upper surface of the filter were removed using a cotton swab.
To elucidate the underlying mechanism of tube expansion failure in COPI-deficient PSG, we first stained the dsRNA-treated PSG cryostat sections.
Cells were first stained with 5 µM CMFDA in cell culture medium for 30 min., at 37°C and 5% CO2.
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