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A first source of miRNA regulated genes consisted of empirically shown interactions of miRNA with genes.
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To reduce the impact of a possible research bias on the results, a second source of miRNA regulated genes was added from a computational prediction of miRNA regulated genes.
Facing circulating miRNAs as cancer biomarker molecules, the first source of variability to be considered is related to the tumor itself.
The data from all eligible studies were extracted by two investigators independently, which included first authors' surname, publication year, origin of population, sample number, tumor type, follow-up period, first-line therapies, source of miRNA, miR-375 assessment methods and the cut-off definition, and HR of miR-375 expression for OS as well as 95% corresponding interval (CI) and P value.
Always have a second source of money.
Second, the abundant fortuitous foldbacks in the genomes can also supply as a source of miRNA genes [16].
Finally, nucleotide additions at the 3'-end of the miRNA are another source of miRNA variability [ 21- 23].
As the precise source of miRNA in plasma is not yet known, there was particular interest in comparing the expression between plasma and cellular miRNA patterns.
The authors measured miRNAs from PF supernatant for the first time, raising the possibility of using PDE from PD patients as a source of miRNAs that could be used as biomarkers to monitor PD therapy [ 50].
Many pioneering studies describing specific miRNA patterns in cardiovascular diseases used plasma as their source of miRNAs.
Second, sources of discomfort were not assessed.
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