Starting in late July, the ship began her first round of target duty, first for the cruisers of the Pacific Fleet, and then for the battleship Nevada.
"We now take a third round of targets that are the best in the G20, the best of any comparable developed country on a per capita basis.
The SPR epitope mapping data served as a basis for a second round of targeted Ala mutations.
It is likely that many more proteins of lower abundance could have been quantified in a second round of targeted mass spectrometric analysis, by actively avoiding the peptides analyzed in the first round.
The presence of the puromycin cassette flanked by two Lox-P sites allowed us to select the targeted cells in the first round followed by Cre recombinase-mediated excision of the puromycin cassette and a second round of targeting using the same pFSVpur-LoxP-TC-I4 construct.
Jessica Jackson Sloan, the national director of Cut 50, told VICE News she thought legislation altering sentences for violent offenders would be feasible "down the road," if not in the first round of bills targeting criminal justice reform.
After identification of the correctly targeted allele the SEPT element was excised using Cre recombinase and its removal was verified by the absence of a PCR product when using primers A and B. This first round of gene targeting resulted in the generation of cell lines that only have a single copy of the wild-type LSD1 (heterozygous), with only one LoxP site flanked by HA1 and HA2.
Playing with frightening precision and total disregard for some difficult pin positions, Woods shot a 10-under-par 62, breaking his own course and tournament record of 63 that he had in the first round of the 2002 Target.
The strategy for LIC-based cloning of inverted repeat constructs is outlined in Figure 2. In the first round of PCR the target sequence is amplified with two pairs of primers to generate sense and antisense products.
Defeated in the first round of the polls, Sankoh targeted civilians.
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