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The simplicity of this approach makes it an appealing choice for conducting a first round of analyses.
Thus, at the end of the first round of analyses, the results from the two techniques in a total of eight cases were discordant.
Results that were statistically significant in the first round of analyses but non-significant when excluding the neocortex volumes (or vice versa) have to be judged carefully (Additional files 5 and 6).
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A second round of analyses was still performed, in which only those samples including 20 individuals at least were selected.
A second round of analyses using an independent series of controls (CG2) could not, however, confirm the initial finding, and instead suggested another mtSNP candidate with a marginal significant P-value, namely A4769G.
In the second round of analyses, this fact was explained by the presence of intratumoural heterogeneity.
In the second round of analyses homozygotes for the minor allele were grouped together with heterozygotes.
This second round of analyses was performed in different rooms and by different individuals.
The results were independently scored by two observers and a second round of analyses confirmed the results.
A second round of analyses was then performed by limiting the dataset to a set of selected sequences representative of the main phylogenetic trends observed.
At the SNP markers, homozygotes for the minor allele were grouped together with heterozygotes in the second round of analyses, i.e. DRD2 A1+ group consisted of A1/A1 (TT) and A1/A2 (CT) genotypes, DRD2 B+ group consisted of B1/B1 (AA) and B1/B2 (AG) genotypes.
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CEO of Professional Science Editing for Scientists @ prosciediting.com