Sentence examples for first reaction with from inspiring English sources
Next, cRNA was produced from this first reaction with T7 RNA polymerase, which simultaneously amplifies target material and incorporates cyanine 3-labeled CTP.
The second reaction used 1 μL of PCR product from the first reaction, with the same proportion of reagents.
Pleasingly, our first reaction with Co acac 3/TMEDA, 19 gave 11 in 20 % yield (Table 1, entry 10) with an improved selectivity for trans- 11.
For gradient titration experiments, 5 μg of gradient protein was used in the first reaction, with three-fold increase in subsequent reactions (i.e. 15, 45 μg and so on).
We performed the first reaction with the outer primer pair (polFouter and polRouter) of a nested PCR method, targeting the viral DNA polymerase gene with highly degenerate consensus primers that have been described recently (5 ).
The first reaction with the primer (0168-2 or Pd1-3) of insert site and primer (1609NdeI, 1609R or far-1) for the inserted gene verified the genes were inserted in the correct orientation.
Although reactions are removed in the order of their flux range (smallest range first), reactions with identical flux ranges may exist (e.g., from parallel pathways) from which then one is chosen randomly.
Following this reaction, one of these wells was then processed for a second reaction with hypocretin (rabbit anti HCRT sc8070, 1∶500, Santa Cruz Biotechnology, Santa Cruz, CA) and the other well for melanin concentrating hormone (Rabbit anti MCH, H-070-47, 1∶5K, Phoenix Pharmaceuticals Inc ,Burlingame, CA).
Amplifications followed previously-published methods [6], [36], [37], except that a MasterAmp kit (Epicentre Biotechnologies) was used for some 28S reactions, and some EF-1α reactions were conducted in a nested fashion, using amplification products from an initial reaction in a second reaction with higher annealing temperatures.
The second reaction with primers (0168-1/0168-2 of pD1-1/p pD1-2d-2) of inserted site verified the wild-type was replaced completely.
A second reaction with primary antibodies to CX3CR1 (dilution 1 : 50, 1 h at RT in the dark) was performed.
