Exact(45)
The three multiplex PCRs of one sample were pooled during the first purification step.
This is the first purification of an enzyme that catalyzes N-linked phosphorylation in proteins.
a, Analysis of the first purification step (Ni2+-affinity chromatography) of TcA WT) and sensor-loop deletion variants.
Performing each reaction step on the same AMPure XP beads used for first purification after end repair minimizes sample losses during the successive clean up steps.
Considering that hTSH is highly hydrophobic and elutes only with the addition of organic solvents, hydrophobic interaction chromatography was adopted as the first purification step; this resulted in the elimination of, among others, the major contaminant.
(A) The first purification step used Superdex 200.
Similar(15)
After cleavage, a buffer exchange was performed to remove DTT prior to the second purification step.
Low efficiencies and week-to-month-long protocols often give rise to mixed cultures requiring a second purification step.
A second purification step, based on size exclusion chromatography, was then utilized, being effective in the elimination of other previously identified contaminating proteins.
Second purification was performed using DyeEX Kit (QIAGEN Inc., USA).
Activated alumina was used as a second purification step.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com