Sentence examples for first perfused from inspiring English sources
Livers were first perfused with perfusion medium (Gibco, Waltham, MA, USA, cat#17701-038) for 10 minutes at 5 ml/min.
For this purpose, the probes were first perfused with a L-DOPA solution (10, 100 or 200 ng/ml in the perfusion fluid for the 10, 25 and 50 mg/kg dose groups, respectively) for 60 min to collect 6 fractions.
For hepatic microsomes, liver was first perfused with 0.9% sodium chloride solution and excised out.
In this work, ZFO nanocrystals were first perfused into pristine TONTA pipelines using a bias voltage.
Lungs were first perfused with 10% formalin at constant distending pressure of 25 cm H2O for 10 min. Liver and lung were excised from the animals, and then placed in 10% formalin overnight at 4°C.
At this stage, liver wedges were first perfused with 'non-recirculating' wash buffer (10 mM 4- 2-hydroxylethyl -1-piperazineethanesulfonic acid (HEPES) pH 7.2) (Sigma, Dorset, UK) at room temperature using a flow rate of 75 ml/min, ensuring flushing out of blood within the liver vasculature.
For brain histological and immunohistochemistry (IHC) stains, anesthetized R6/2 mice and their WT were first brain perfused with phosphate-buffered saline containing 4% formaldehyde.
First, we perfused four animals in a preliminary group with 10 μM BPA-GA for 20 min to determine whether BPA-GA is transferred into the fetus across the placenta.
One was subjected to the anoxia/reoxygenation protocol of Figure 5A, and the second heart was perfused with oxygen-containing perfusate for an equivalent time (1 hr at 1.5 ml/min; 37°C).
In brief, the hepatic portal vein was ligated and perfused first with HBSS containing 5 mM EDTA without Ca2+ and Mg2+ (Beyotime Co., China), then with HBSS containing 0.025% type IV collagenase (Sigma-Aldrich).
Briefly, a venular microvessel in rat mesentery was cannulated and perfused first with albumin-Ringer solution containing 10 µM fura 2-AM for 45 min. The vessel was then recannulated and perfused with albumin-Ringer solution for 10 min to remove fura 2-AM from the lumen.
