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We grew the cells in floating mammosphere conditions before the first passage for several reasons.
Cells were cultured and frozen down from the first passage for future use.
Most of the mice inoculated with the 3 bovine TSE isolates were positive (28/31 at first passage) for PrPres by Western blot or PrPd by immunohistochemical testing.
The mean time to the first passage for BDIM (1.1), m j, n) = E(T j, n)), can be calculated using the formula m j, n) = m0 j, n) + m1 j, n).
CPE was observed in rhabdomyosarcoma cells in the first passage for 33 samples, of which 27 were pan-EV positive, identified as E13 (21 [64%]), E7 (2 [6%]), E4 (1 [3%]), E33 (1 [3%]), and EV75 (2 [6%]); 6 isolates remained untypeable.
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The survival period at second passage for L-type BSE was considerably reduced and significantly shorter (>120 days) than typical BSE after 2 passages into TgOvPrP4 mice.
The comparatively longer IP generated by that goat sample relative to these mouse-adapted BSE isolates is a common observation at second passage; for example, the IP of the serially passaged ovine BSE at second passage was 148 ± 3 days postinoculation.
The results of our analyses demonstrate that the K/Na ratio was different for each cell passage (4.72 for the first passage, 4.55 for the second passage, and 7.82 for the third passage), suggesting that the highest cell viability corresponds to the third passage.
The first passage problem for linear and non-linear oscillators excited by white and coloured noise are considered.
The inverse Gaussian distribution is used to describe the first passage time for a particle (moving with constant velocity) that is subject to linear Brownian motion.
A method is also proposed to calculate the time-dependent first passage probability for the case where wave climate and mean water depth change with time.
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